Application
Western blot analysis of RCE1 expression in Hep G2 whole cell lysate.| Product Name | RCE1 Antibody (T-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RCE1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | RCE1, a ubiquitously expressed, integral membrane endoprotease, facilitates membrane targeting of Ras and other prenylated proteins by releasing the last three carboxy-terminal amino acids, AAX, of the CAAX motif. Human RCE1 activity is specific for prenylated, farnesylated, and geranylated proteins such as K-RAS, N-RAS, H-RAS, RAP1B and G-GAMMA-1. Inhibition of RCE1 leads to retardation of cell growth, limitation of Ras-induced cell transformation and sensitization of tumor cells to a farnesyltransferase inhibitor. RCE1 belongs to the U48 peptidase family. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RCE1 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of RCE1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 9986 |
|---|
Application
Western blot analysis of RCE1 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
