Application
Western blot analysis of OC-2 expression in Jurkat whole cell lysate.| Product Name | OC-2 Antibody (W-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of OC-2 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | The predicted 485 amino acid ONECUT2 (OC-2) protein is a transcriptional activator that functions in activating the transcription of several liver genes, such as HNF3-∫. OC-2 is a member of the CUT homeobox family and contains one CUT DNA-binding domain and one homeobox DNA-binding domain. OC-2 shares several regions of sequence homology with OC-1 (HNF6), including a serine/threonine- and proline-rich sequence (STP box). OC-2 localizes to the nucleus and abundant expression of OC-2 is observed in liver and skin tissues, whereas lower expression is demonstrated in testis, brain (occipital cortex) and urinary bladder tissues. The ability of OC-2 to recognize binding sites present in regulatory regions of liver-expressed genes differ from, but overlap with, those of OC-1. Like OC-1, recombinant OC-2 stimulates transcription of the HNF3-∫ gene. Research also suggests that OC-2 participates in liver differentiation and metabolism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of OC-2 of mouse and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of OC-2 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 9480 |
|---|
Application
Western blot analysis of OC-2 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
