Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Crk II expression in non-transfected: (A) and mouse Crk II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatocytes and bile duct cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | Crk II Antibody (H-53) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 190-242 of Crk II of human origin |
| Modification Notes | The Crk family of adapter proteins including Crk-II, Crk-I, and Crk-L consist mostly of SH2 and SH3 domains (1). Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline-rich motifs, they are involved in a variety of signaling cascades (1). Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities (2-4). Crk-II has less transforming activity than Crk-I, although both Crk-I and Crk-II bind to many tyrosine-phosphorylated proteins that bind to grb2 (2,4). In addition, Crk-II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor I (IGF-I) and might be involved in the IGF-I receptor signalling pathway (5). The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Crk II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 190-242 of Crk II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 190-242 of Crk II of human origin |
| Entrez Gene | 1398 |
|---|
Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Crk II expression in non-transfected: (A) and mouse Crk II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Crk II expression in non-transfected: (A) and human Crk II transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatocytes and bile duct cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of Crk II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
