Application
Western blot analysis of PAP-2a expression in Hep G2 whole cell lysate.| Product Name | PAP-2a Antibody (A-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PAP-2a of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | PAP-2 (phosphatidic acid phosphatase 2), also known as lipid phosphate phosphohydrolase (LPP), is a family of integral membrane glycoproteins that dephosphorylate a variety of lipid phosphates and play a role in signal transduction via the phospholipase D pathway. PAP-2 proteins function independently of Mg2+ and are insensitive to NEM (N-ethylmaleimide) inhibition. The lipid phosphates degraded by this family include ceramide 1-phosphate (C1P), sphingosine 1-phosphate (S1P), phosphatidic acid (PA) and lysophosphatidic acid (LPA). There are three PAP-2 isozymes: PAP-2a, PAP-2b and PAP-2c (also known as LPP1, LPP3 and LPP2 respectively). PAP-2a and PAP-2b are ubiquitously expressed and most effectively hydrolyze PA and LPA. PAP-2c is predominantly expressed in human brain, placenta and pancreas, and in mouse liver, lung and kidney. PAP-2c most effectively hydrolyzes LPA and S1P and may function as a cell cycle regulator. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PAP-2a of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PAP-2a of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 8611 |
|---|
Application
Western blot analysis of PAP-2a expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
