Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing membrane and cytoplasmic staining of squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of IL-3Rα expression in MCP-5 whole cell lysate.| Product Name | IL-3Rα Antibody (V-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of IL-3Rα of mouse origin |
| Modification Notes | Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific å subunit (IL-3Rå) and a signal transducing ∫ subunit (IL-3R∫) common to the IL-3/IL-5/GM-CSF receptors. The å subunits are low-affinity ligand-binding proteins while the ∫ subunits do not themselves bind ligand, but are required for high affinity binding by the å subunits. The mouse IL-3 receptor has two distinct ∫ subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine ∫ subunits are 91% homologous at the amino acid level but only 56% homologous to the human ∫ subunit. The carboxy-terminus of the ∫ subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IL-3Rα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of IL-3R&alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of IL-3Rα of mouse origin |
| Entrez Gene | 3563 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing membrane and cytoplasmic staining of squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of IL-3Rα expression in MCP-5 whole cell lysate.| Application Notes | recommended for detection of IL-3Rα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
