Application
Western blot analysis of RoXaN expression in human liver tissue extract.| Product Name | RoXaN Antibody (X-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of RoXaN of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | RoXaN (zinc finger CCCH-type containing 7B), is a 993 amino acid protein likely involved in translational regulation whose alternative names include rotavirus 'X'-associated non-structural protein, ubiquitous tetratricopeptide containing protein RoXaN, ZC3H7B, FLJ13787, KIAA1031, or DKFZp434K0920. RoXaN localizes to the nucleus and has been identified as a novel cellular protein-binding partner for the rotavirus nonstructural protein NSP3, which is involved in rotavirus replication. RoXaN’s nuclear localization is known to appear disguised upon infection with rotavirus A. Two RoXaN isoforms exist as a result of alternative splicing, and RoXaN also contains one C2H2-type zinc finger, three TPR repeats and four C3H1-type zinc fingers. RoXaN contains one paxillin leucine-aspartate repeat (LD) motif in addition to a coiled-coil region which assist in protein-protein interactions. The gene encoding RoXaN maps to human chromosome 22q13.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RoXaN of human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of RoXaN of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 23264 |
|---|
Application
Western blot analysis of RoXaN expression in human liver tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
