Application
Western blot analysis of CSDA expression in Jurkat (A) and Hep G2 (B) whole cell lysates and human fetal muscle tissue extract (C).| Product Name | CSDA Antibody (F-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CSDA of human origin by WB, IP and ELISA |
| Modification Notes | CSDA (cold shock domain protein A), also known as DBPA, CSDA1 or ZONAB (zonula occludens 1-associated nucleic acid-binding protein), is a 372 amino acid nuclear and cytoplasmic protein that is highly expressed in skeletal muscle and heart. Containing one CSD (cold-shock) domain, CSDA is thought to bind to GM-CSF promoter, full length mRNA and to short RNA sequences containing a specific consensus site. CSDA is suggested to have a role in translation repression and is found in a mRNP complex with MSY2. MSY2 belongs to the Y-box family of multifunctional proteins that regulate both transcription and translation. CSDA participates in promoting cell proliferation and expression of cyclin D1 and proliferating cell nuclear antigen (PCNA). CSDA is regarded to be an important component of the mechanisms that sense epithelial density and in regulating the switch between proliferation and differentiation through complex transcriptional networks. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CSDA of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of CSDA of human origin by WB, IP and ELISA |
| Entrez Gene | 8531 |
|---|
Application
Western blot analysis of CSDA expression in Jurkat (A) and Hep G2 (B) whole cell lysates and human fetal muscle tissue extract (C).| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
