Application
Western blot analysis of PHF20 expression in Jurkat whole cell lysate.Application
Western blot analysis of PHF20 expression in non-transfected 293T: (A), human PHF20 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | PHF20 Antibody (G-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PHF20 of human origin by WB, IP and ELISA |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. PHF20 (PHD finger protein 20), also known as NZF, TZP, GLEA2 or HCA58, is a 1,012 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger, one C2H2-type zinc finger and one A/T hook DNA-binding domain. Expressed in a variety of tissues, including liver, heart, lung, pancreas, spleen, testis and placenta, PHF20 is thought to function as a transcription factor, possibly playing a role in carcinogenesis. PHF20 exists as two alternatively spliced isoforms which are encoded by a gene that maps to human chromosome 20. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PHF20 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PHF20 of human origin by WB, IP and ELISA |
| Entrez Gene | 51230 |
|---|
Application
Western blot analysis of PHF20 expression in Jurkat whole cell lysate.
Application
Western blot analysis of PHF20 expression in non-transfected 293T: (A), human PHF20 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
