Application
Immunofluorescence staining of methanol-fixed MIA PaCa-2 cells showing cytoplasmic localization.Application
Western blot analysis of TRAP-1 expression in Hep G2 (A), A-673 (B) and MIA PaCa-2 (C) whole cell lysates.| Product Name | TRAP-1 Antibody (H-350) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 510-860 mapping at the C-terminus of TRAP-1 of human origin |
| Modification Notes | Transforming growth factor b (TGF∫) receptor associated binding protein (TRAP-1) participates in the regulation of the TGF∫ signaling pathway. TGF∫ is a secreted ligand that induces transcription of various targeted genes involved in cell proliferation, differentiation and apoptosis. This is done by sequentially binding to surface TGF∫ type II receptors and inducing the auto-phosphorylation of the type II receptor and the transient transactivation of the type I TGF∫ receptor. The signal is then propagated through the Smad family of transcription factors, which leads to the expression of the targeted genes. The cytosolic TRAP-1 protein selectively associates with the phosphorylated type I TGF∫ receptors, but not with the unphosphorylated type I- or type II-receptors. TRAP-1 binding to the receptor results in the inhibition of TGF∫ signaling, thereby inhibiting the transcription of TGF∫ target genes. The carboxy-terminus of TRAP-1 is also able to bind to 5-lipoxygenase, a mediator of lipid metabolism for the production of leukotrienes, where it may then regulate the signaling within leukocytes and other inflammatory mediating cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRAP-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 510-860 mapping at the C-terminus of TRAP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 510-860 mapping at the C-terminus of TRAP-1 of human origin |
| Entrez Gene | 9392 |
|---|
Application
Immunofluorescence staining of methanol-fixed MIA PaCa-2 cells showing cytoplasmic localization.
Application
Western blot analysis of TRAP-1 expression in Hep G2 (A), A-673 (B) and MIA PaCa-2 (C) whole cell lysates.| Application Notes | recommended for detection of TRAP-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
