Application
Western blot analysis of FTβ expression in non-transfected 293T: (A), human FTβ transfected 293T: (B), A-431 (C) and JAR (D) whole cell lysates.Application
Western blot analysis of FTβ expression in 3611-RF (A) and A-431 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.| Product Name | FTβ Antibody (X-28) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of FTβ of rat origin |
| Modification Notes | Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical å and ∫ subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins (1). The natural substrates contain the Cys-A-A-Xaa recognition sequence (2), where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine (3,4). The purified farnesyl transferase is an a-b heterodimer (1,5). The ∫ subunit, which is known as FT∫, CAAX farnesyltransferase subunit ∫, or Ras proteins prenyltransferase subunit ∫, is a 437 amino acid protein that contains five PFTB repeats and binds the peptide substrate (6). The å subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FTβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of FT&beta of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of FTβ of rat origin |
| Entrez Gene | 2342 |
|---|
Application
Western blot analysis of FTβ expression in non-transfected 293T: (A), human FTβ transfected 293T: (B), A-431 (C) and JAR (D) whole cell lysates.
Application
Western blot analysis of FTβ expression in 3611-RF (A) and A-431 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.| Application Notes | recommended for detection of FTβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
