Application
Western blot analysis of ARK5 expression in non-transfected: (A) and human ARK5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARK5 expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | ARK5 Antibody (N-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ARK5 of human origin and Nuak1 of mouse and hamster origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | 5’-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Activated AMPK phosphorylates and regulates hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The AMPK-related kinase 5 (ARK5) is a tumor progression-associated factor that is directly phosphorylated by Akt at Ser 600, which is in its regulatory domain. NDR2, a serine/threonine protein kinase, phosphorylates and activates ARK5 during Insulin-like growth factor (IGF)-I signaling in order to promote cell survival. This activation may be involved in the invasion of colorectal cancer cell lines. Furthermore, ARK5 is a transcriptional target of the Large-MAF family and may have a role in mediating the aggressiveness of c-Maf- and MafB-expressing myelomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ARK5 of human origin and Nuak1 of mouse and hamster origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ARK5 of human origin and Nuak1 of mouse and hamster origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9891 |
|---|
Application
Western blot analysis of ARK5 expression in non-transfected: (A) and human ARK5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARK5 expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
