Application
Western blot analysis of epsin 1 expression in non-transfected 293T: (A), mouse epsin 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of epsin 1 expression in non-transfected 293T: (A), human epsin 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of epsin 1 expression in non-transfected 293T: (A), mouse epsin 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing luminal membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of epsin 1 expression in Jurkat whole cell lysate.Application
Western blot analysis of human recombinant epsin 1 fusion protein.Application
Western blot analysis of epsin 1 expression in HeLa whole cell lysate.| Product Name | epsin 1 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 311-440 mapping to an internal region of epsin 1 of human origin |
| Modification Notes | Epsin 1 (EPN1) is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the å subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both Epsin and Eps15, like other cytosolic components of the synaptic vesicle endo-cytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an Epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of epsin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 311-440 mapping to an internal region of epsin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 311-440 mapping to an internal region of epsin 1 of human origin |
| Entrez Gene | 29924 |
|---|
Application
Western blot analysis of epsin 1 expression in non-transfected 293T: (A), mouse epsin 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of epsin 1 expression in non-transfected 293T: (A), human epsin 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of epsin 1 expression in non-transfected 293T: (A), mouse epsin 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing luminal membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of epsin 1 expression in Jurkat whole cell lysate.
Application
Western blot analysis of human recombinant epsin 1 fusion protein.
Application
Western blot analysis of epsin 1 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of epsin 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
