Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of A cyclase II expression in 293T (A) and SH-SY5Y (B) whole cell lysates.| Product Name | A cyclase II Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of A cyclase II of rat origin |
| Modification Notes | Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs coupled receptor catalyzes the exchange of GDP (bound to Ga s) for GTP, the dissociation of GTP-Ga s from Gbg and Ga s-mediated activation of adenylyl cyclase. Adenylyl cyclases of the type II family differ from other subforms in that they are conditionally stimulated by Ga s/bg subunits and regulated by PKC-mediated C-terminal phosphorylation. Both short- and long-term activation of D(2L) dopamine receptors result in a marked degree of sensitization of A cyclase I, II, V and IX, but not A cyclase VIII. The effects on A cyclase I, II and VIII is dependent upon the ability of these A cyclase isoforms to synergistically respond to selective activators in the presence of activated Ga s. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of adenylyl cyclase II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of A cyclase II of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of A cyclase II of rat origin |
| Entrez Gene | 108 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of A cyclase II expression in 293T (A) and SH-SY5Y (B) whole cell lysates.| Application Notes | recommended for detection of adenylyl cyclase II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
