Application
Western blot analysis of Gab 1 expression in non-transfected: (A) and mouse Gab 1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Gab 1 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of Gab 1 expression in non-transfected: (A) and human Gab 1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Gab 1 expression in K-562 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic staining.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.| Product Name | Gab 1 Antibody (H-198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 119-316 of Gab 1 of human origin |
| Modification Notes | Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity (1-3,8). These are immediate substrates of receptor tyrosine kinase activity and serve to link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate (4-7). Shc and Gab 1 also participate in insulin signaling by linking the insulin receptor to Ras by forming complexes with GRB2 (another adapter protein) and Sos independently of IRS-1. Gab 1 is also thought to be involved in the EGF receptor signaling pathway (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Gab 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 119-316 of Gab 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 119-316 of Gab 1 of human origin |
| Entrez Gene | 2549 |
|---|
Application
Western blot analysis of Gab 1 expression in non-transfected: (A) and mouse Gab 1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Gab 1 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of Gab 1 expression in non-transfected: (A) and human Gab 1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Gab 1 expression in K-562 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic staining.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic staining.| Application Notes | recommended for detection of Gab 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
