Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of GRB7 expression in non-transfected: (A) and mouse GRB7 transfected: (B) 293T whole cell lysates.| Product Name | GRB7 Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of GRB7 of human origin |
| Modification Notes | Many growth factors function by binding receptors with intrinsic tyrosine kinase activity (1,2). Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and the phosphotyrosine-containing receptor sequences (3,4). GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N-terminus (5,6). GRB7 maps to the region on mouse chromosome 11 containing the Neu gene (6). This region of mouse chromo-some 11 is syntenic to an area of human chromosome 17q that is frequently amplified in breast cancer (6,7). Moreover, GRB7 is amplified and overexpressed in breast cancer and is found in a complex with Neu gp185 (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GRB7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of GRB7 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of GRB7 of human origin |
| Entrez Gene | 2886 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of GRB7 expression in non-transfected: (A) and mouse GRB7 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of GRB7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
