Application
Western blot analysis of SPPL2b expression in Jurkat whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Product Name | SPPL2b Antibody (F-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against SPPL2b of human origin |
| Modification Notes | SPPL2b (signal peptide peptidase-like 2b), also known as PSL1 or IMP4 (intramembrane protease 4), is a 592 amino acid multi-pass membrane protein that contains one protease associated domain and belongs to the GXGD family of aspartic proteases. Localized to the membrane of the Golgi apparatus and to lysosomes, endosomes and the plasma membrane, SPPL2b functions as an intramembrane protease that specifically cleaves the transmembrane domain of TNFå. Once cleaved, TNFå releases its intracellular domain, thus triggering the immunity-related expression of cytokines (also known as interleukins) which are used in various pathways throughout the body. SPPL2b also interacts with and catalyzes the intramembrane proteolysis of ITM2B (integral membrane protein 2B), a protein that, when mutated, is associated with dementia. Four isoforms of SPPL2b exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SPPL2b of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against SPPL2b of human origin |
|---|---|
| Tissue Specificity | raised against SPPL2b of human origin |
| Entrez Gene | 56928 |
|---|
Application
Western blot analysis of SPPL2b expression in Jurkat whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Application Notes | recommended for detection of SPPL2b of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
