Application
Western blot analysis of RBM22 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human muscle tissue showing nuclear staining.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear staining.| Product Name | RBM22 Antibody (B-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against RBM22 of human origin |
| Modification Notes | The RBM (RNA-binding motif) gene family encodes proteins with an RNA binding motif that have been suggested to play a role in the modulation of apoptosis. RBM22, also designated Zinc finger CCCH domain-containing protein 16, is a highly conserved RNA binding protein that is predominantly expressed in spleen and is localized to the nucleus. With a RRM (RNA recognition motif domain) and a C3H1-type zinc finger, RBM22 is primarily involved in pre-mRNA splicing. In the presence of RBM22, cytosolic ALG-2 (Apoptosis Linked Gene 2) translocates to the nucleus, suggesting a functional interaction between the two proteins. Homologs of RBM22 are essential proteins in the regulation of alternative splicing in the cell cycle, zebrafish development and Drosophilia heart development. The gene encoding RBM22 is significantly downregulated in patients with the 5q deletion syndrome, a clonal disease of the hematopoietic stem cell in which characteristic changes in megakaryocytes result in treatement-resistant anemia and myelodysplastic syndromes that may eventually lead to acute myelogenous leukemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RBM22 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against RBM22 of human origin |
|---|---|
| Tissue Specificity | raised against RBM22 of human origin |
| Entrez Gene | 55696 |
|---|
Application
Western blot analysis of RBM22 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human muscle tissue showing nuclear staining.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear staining.| Application Notes | recommended for detection of RBM22 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
