Application
Western blot analysis of TC-PTP expression in Jurkat whole cell lysate.| Product Name | N-twist Antibody (O-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against N-twist of human origin |
| Modification Notes | N-twist (Neuronal twist), also known as Fer3-like protein or Nephew of atonal 3, is a 166 amino acid member of the basic helix–loop–helix (bHLH) transcription factor family. N-twist functions as an inhibitor of transcription by binding DNA at the E box, a consensus bHLH-binding site. Primarily expressed in the developing central nervous system, N-twist interacts with the bHLH domain of E12 to form a heterodimer. By sequestering E proteins in a dominant-negative fashion, N-twist inhibits ASH1L-dependent transcriptional activation, which suggests the role N-twist may play in the negative regulation of neurogenesis. Methylation of the gene encoding N-twist has shown to be associated with a poor prognosis in CpG island (CGI) methylator phenotype (CIMP) positive neuroblastomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of N-twist of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against N-twist of human origin |
|---|---|
| Tissue Specificity | raised against N-twist of human origin |
| Entrez Gene | 222894 |
|---|
Application
Western blot analysis of TC-PTP expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of N-twist of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
