Application
Western blot analysis of Stat5a/b phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) HeLa whole cell lysates.Application
Western blot analysis of phosphorylated Stat5a/b expression in untreated (A) and EGF-treated (B) HeLa whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-Stat5a/b Antibody (Tyr 694) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 694 of Stat5a/b of human origin |
| Modification | Phosphoralated |
| Modification Notes | Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2) (1). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines (2–4). IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively (2,5). Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a (2,5). Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level (2). Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response (3). Ser779 is constitutively phosphorylated in the mammary gland, and Ser725 phosphorylation influences prolactin-stimulated in vitro DNA binding activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 694 phosphorylated Stat5a and Tyr 699 phosphorylated Stat5b of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 694 of Stat5a/b of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 694 of Stat5a/b of human origin |
| Entrez Gene | 6776 |
|---|
Application
Western blot analysis of Stat5a/b phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) HeLa whole cell lysates.
Application
Western blot analysis of phosphorylated Stat5a/b expression in untreated (A) and EGF-treated (B) HeLa whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Tyr 694 phosphorylated Stat5a and Tyr 699 phosphorylated Stat5b of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
