Application
Antibodies tested include p-p53 (Ser 315): (A,B,C) and p53 (Pab 240): (D,E,F).Application
Antibodies tested include p-p53 (Ser 315): (A) and p-p53 (Ser 315): preincubated with cognate phosphorylated peptide (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-p53 Antibody (Ser 315) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 315 of p53 of human origin |
| Modification | Phosphoralated |
| Modification Notes | p53 is a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers. Phosphorylation of p53 at residue Thr 155 is mediated by the COP9 signalosome (CSN) and targets p53 to ubiquitin-26S Proteasome-dependent degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 315 phosphorylated p53 of h origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 315 of p53 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 315 of p53 of human origin |
| Entrez Gene | 7157 |
|---|
Application
Antibodies tested include p-p53 (Ser 315): (A,B,C) and p53 (Pab 240): (D,E,F).
Application
Antibodies tested include p-p53 (Ser 315): (A) and p-p53 (Ser 315): preincubated with cognate phosphorylated peptide (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 315 phosphorylated p53 of h origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
