Application
Blots were probed with p-HDAC5 (Ser 498): preincubated with cognate phosphorylated peptide (A) and p-HDAC5 (Ser 498): (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-HDAC5 Antibody (Ser 498) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 498 of HDAC5 of human origin |
| Modification | Phosphoralated |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acet-ylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, p300/CBP, PCAF (p300/CBP-associated factor), HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 498 phosphorylated HDAC5 of human origin and correspondingly phosphorylated Ser 488 of mouse origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 498 of HDAC5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 498 of HDAC5 of human origin |
| Entrez Gene | 10014 |
|---|
Application
Blots were probed with p-HDAC5 (Ser 498): preincubated with cognate phosphorylated peptide (A) and p-HDAC5 (Ser 498): (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 498 phosphorylated HDAC5 of human origin and correspondingly phosphorylated Ser 488 of mouse origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
