Application
Western blot analysis of ACYP2 expression in Sol8 whole cell lysate.| Product Name | ACYP2 Antibody (FL-99) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-99 representing full length acylphosphatase of human origin |
| Modification Notes | The formation of stable highly organized protein aggegrates, known as amyloid fibrils, is associated with several debilitating human diseases, including Alzheimer’s disease, Parkinson’s disease, and Creutzfeldt-Jakob disease (1,2). In each of these conditions, a peptide or protein that is normally soluble accumulates into insoluble fibrils (1,2). Muscle acylphosphatase (AcP) has emerged as a significant model system to study protein misfolding and aggregation (1,3-6). It is particularly suitable for these studies because muscle AcP is a small, simple protein of only 98 amino acids consisting of a five-stranded antiparallel b-sheet and two parallel a-helices (1). Mutations in the muscle AcP between residues 16-31 and 87-98, which includes its phosphate binding site at Arg-23, significantly increases the rate of aggregation (1,6). These mutations correlate with changes in the hydrophobicity of AcP and a conversion of the a-helical structures to b-sheets (1). Therefore, a reduction in the net charge of a protein may be a key determinant in some forms of protein deposition diseases (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of acylphosphatase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-99 representing full length acylphosphatase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-99 representing full length acylphosphatase of human origin |
| Entrez Gene | 98 |
|---|
Application
Western blot analysis of ACYP2 expression in Sol8 whole cell lysate.| Application Notes | recommended for detection of acylphosphatase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
