Application
Western blot analysis of GluR-1 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse cerebellum tissue extracts.Application
Western blot analysis of GluR-1 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.Application
Blots were probed with p-GluR-1 (Ser 849): preincubated with cognate phosphorylated peptide (A) and p-GluR-1 (Ser 849): (B).| Product Name | p-GluR-1 Antibody (Ser 849) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 849 of GluR-1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate, whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca++ ions. The NMDA receptors consist of five subunits: four epsilion subunits (epsilon 1, 2, 3 and 4) and one zeta subunit. The zeta subunit is expressed throughout the brainstem whereas the four epsilon subunits display limited distribution. Serine 831 is specifically phosphorylated by CaM kinase II and is the major site of CaM kinase II phosphorylation on GluR-1. In addition, treatment of hippocampal slice preparations with phorbol esters and forskolin increase the phosphorylation of Serine 831 and 845, respectively, indicating that protein kinase C and protein kinase A phosphorylate these residues in hippocampal slices. GluR-1 phosphorylation is critical for synaptic plasticity, and that identical stimulation conditions recruit different signal-transduction pathways depending on synaptic history. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Ser 849 phosphorylated GluR-1 of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 849 of GluR-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 849 of GluR-1 of human origin |
| Entrez Gene | 2890 |
|---|
Application
Western blot analysis of GluR-1 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse cerebellum tissue extracts.
Application
Western blot analysis of GluR-1 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.
Application
Blots were probed with p-GluR-1 (Ser 849): preincubated with cognate phosphorylated peptide (A) and p-GluR-1 (Ser 849): (B).| Application Notes | recommended for detection of Ser 849 phosphorylated GluR-1 of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
