Application
Western blot analysis of phosphorylated Integrin β3 expression in untreated (A) and H2O2-treated (B) HL-60 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane localization.| Product Name | p-Integrin β3 Antibody (Tyr 747) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a short amino acid sequence containing phosphorylated Tyr 747 of Integrin β3 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 å and 8 ∫ subunits heterodimerize to produce more than 20 different receptors (1,2). Most integrin receptors bind ligands that are components of the extracellular matrix, but can also bind to soluble ligands or to counterreceptors on adjacent cells, thereby leading to aggregation of cells (2). Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors (3). In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis (4,5). GRB2 binds Integrin ∫3 only when both Tyr 747 and Tyr 759 are phosphorylated (6). However, SHC binds the monophosphorylated Integrin ∫3 at Tyr 759, suggesting that tyrosine phosphorylation of Integrin ∫3 may be important in intiating outside-in signaling cascades by inducing association of signaling components directly with Integrin (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 747 phosphorylated Integrin β3 of mouse and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a short amino acid sequence containing phosphorylated Tyr 747 of Integrin &beta 3 of human origin |
|---|---|
| Tissue Specificity | raised against a short amino acid sequence containing phosphorylated Tyr 747 of Integrin β3 of human origin |
| Entrez Gene | 3690 |
|---|
Application
Western blot analysis of phosphorylated Integrin β3 expression in untreated (A) and H2O2-treated (B) HL-60 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane localization.| Application Notes | recommended for detection of Tyr 747 phosphorylated Integrin β3 of mouse and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
