Application
Western blot analysis of phosphorylated Histone H2A.X expression in untreated (A) and UV-treated (B) 293 whole cell lysates.| Product Name | p-Histone H2A.X Antibody (Ser 139) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 139 of Histone H2A.X of human origin |
| Modification | Phosphoralated |
| Modification Notes | Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. The H2AFX gene is located in close proximity to the Porphobilinogen deaminase (PBG-D) gene in both mouse and human, and maps to chromosome 9 and 11q23, respectively. H2A.X differs from the other members of the H2A family by the presence of a highly conserved C-terminal motif. It is rapidly phosphorylated in response to ionizing radiation and plays an important role in the recognition and repair of DNA double stranded breaks. The phosphorylated form of H2A.X, designated ©-H2A.X, forms nuclear foci at the heavy chain constant region of cells involved in class switch recombination (CSR), a region-specific DNA reaction that replaces one immunoglobulin heavy chain constant region gene with another. The phosphorylated ©-H2A.X is also thought to initiate subsequent repair factors, including Rad50, Rad51 and BRCA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Ser 139 phosphorylated Histone H2A.X of human and mouse origin and correspondingly phosphorylated Ser 140 of rat origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 139 of Histone H2A.X of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 139 of Histone H2A.X of human origin |
| Entrez Gene | 3014 |
|---|
Application
Western blot analysis of phosphorylated Histone H2A.X expression in untreated (A) and UV-treated (B) 293 whole cell lysates.| Application Notes | recommended for detection of Ser 139 phosphorylated Histone H2A.X of human and mouse origin and correspondingly phosphorylated Ser 140 of rat origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
