Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.Application
Immunofluorescence stain-ing of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of FANCL expression in non-transfected: (A) and human FANCL transfected: (B) 293T whole cell lysates.Application
Western blot analysis of FANCL expression in non-transfected 293T: (A), human FANCL transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Product Name | FANCL Antibody (H-197) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 179-375 mapping at the C-terminus of FANCL of human origin |
| Modification Notes | Defects in FANCL are a cause of Fanconi anemia. Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone marrow failure, birth defects and chromosomal instability. At the cellular level, FA is characterized by spontaneous chromosomal breakage and a unique hypersensitivity to DNA cross-linking agents. At least 8 complementation groups have been identified and 6 FA genes (for subtypes A, C, D2, E, F and G) have been cloned. Phosphorylation of FANC (Fanconi anemia complementation group) proteins is thought to be important for the function of the FA pathway. FA proteins cooperate in a common pathway, culminating in the monoubiquitination of FANCD2 protein and colocalization of FANCD2 and BRCA1 proteins in nuclear foci. FANCL is a ligase protein mediating the ubiquitination of FANCD2, a key step in the DNA damage pathway. FANCL may be required for proper primordial germ cell proliferation in the embryonic stage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FANCL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 179-375 mapping at the C-terminus of FANCL of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 179-375 mapping at the C-terminus of FANCL of human origin |
| Entrez Gene | 55120 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.
Application
Immunofluorescence stain-ing of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of FANCL expression in non-transfected: (A) and human FANCL transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of FANCL expression in non-transfected 293T: (A), human FANCL transfected 293T: (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of FANCL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
