Application
Western blot analysis of CAR1/2 expression in mouse liver tissue extract.Application
Western blot analysis of CAR1/2 expression in c4 (A) and EOC 20 (B) whole cell lysates and mouse brain (C) and rat liver (D) tissue extracts.| Product Name | CAR1/2 Antibody (M-127) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The CAR (constitutively acting receptor) proteins, CAR1 and CAR2, are mouse nuclear hormone receptors. CAR1 and CAR2, along with their human homolog, MB67, are in highest expression in the liver and belong to a group of receptors known as orphan receptors due to their lack of a known ligand. Unlike conventional hormone receptors which activate transcription upon binding with steroids, retinoids and thyroid hormones, the CAR and MB67 orphan receptors are transcriptionally active in the absence of exogenous hormone. The CAR and MB67 orphan receptors bind to DNA in the form of a heterodimer with the retinoic-X receptor and activate gene transcription in a constituitive manner. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CAR1 and CAR2 of mouse and rat origin and MB67 of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 70-197 of CAR1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 70-197 of CAR1 of mouse origin |
| Entrez Gene | 9970 |
|---|
Application
Western blot analysis of CAR1/2 expression in mouse liver tissue extract.
Application
Western blot analysis of CAR1/2 expression in c4 (A) and EOC 20 (B) whole cell lysates and mouse brain (C) and rat liver (D) tissue extracts.| Application Notes | recommended for detection of CAR1 and CAR2 of mouse and rat origin and MB67 of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
