Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of HNF-1β expression in non-transfected: (A) and human HNF-1β transfected: (B) 293T whole cell lysates and Hep G2 nuclear extract (C).Application
Western blot analysis of HNF-1β expression in non-transfected: (A) and human HNF-1β transfected: (B) 293T whole cell lysates.| Product Name | HNF-1β Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 6-90 mapping near the N-terminus of HNF-1β of human origin |
| Modification Notes | HNF-1 (a and b), HNF-3 (a, b and g), HNF-4 (a and g) and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3a, HNF-3b and HNF-3g regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3a and HNF-3b have also been shown to be involved in gastrulation events such as body axis formation. HNF-4a and HNF-4g have been shown to be important for early embryo development. HNF-4a is expressed in liver, kidney, pancreas, small intestine, testis and colon; and HNF-4g is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3b, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and a-1 antitrypsin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of HNF-1β of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 6-90 mapping near the N-terminus of HNF-1&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 6-90 mapping near the N-terminus of HNF-1β of human origin |
| Entrez Gene | 6928 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of HNF-1β expression in non-transfected: (A) and human HNF-1β transfected: (B) 293T whole cell lysates and Hep G2 nuclear extract (C).
Application
Western blot analysis of HNF-1β expression in non-transfected: (A) and human HNF-1β transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HNF-1β of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
