Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal and glial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of ERGIC-53 expression in non-transfected: (A) and human ERGIC-53 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ERGIC-53 expression in non-transfected 293T: (A), mouse ERGIC-53 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of ERGIC-53 expression in non-transfected 293T: (A), human ERGIC-53 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of ERGIC-53 expression in HeLa whole cell lysate (A) and truncated human recombinant ERGIC-53 fusion protein (B).| Product Name | ERGIC-53 Antibody (H-245) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Lectin mannose-binding 1, also designated vesicular integral-membrane protein (VIP36) and lectin mannose-binding 2, also designated ER-Golgi intermediate compartment (ERGIC-53) comprise a family of membrane bound, ubiquitous proteins involved in the selective transport of newly synthesized glycoproteins from the endoplasmic reticulum (ER) to the ER-Golgi intermediate compartment (ERGIC). VIP36 acts as an intracellular lectin in the early secretory pathway. It is involved in the sorting and transport of glycoproteins carrying high mannose-type glycans. ERGIC-53, a mannose-specific lectin, recognizes sugar residues of glycoproteins and glycolipids. It mediates the sorting and recycling of proteins and/or lipids. Null expression of ERGIC-53, also designated LMAN1, results in a rare autosomal recessive bleeding disorder that causes combined deficiency of both coagulation factors V and VIII. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ERGIC-53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 266-510 mapping at the C-terminus of ERGIC-53 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 266-510 mapping at the C-terminus of ERGIC-53 of human origin |
| Entrez Gene | 3998 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal and glial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of ERGIC-53 expression in non-transfected: (A) and human ERGIC-53 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ERGIC-53 expression in non-transfected 293T: (A), mouse ERGIC-53 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of ERGIC-53 expression in non-transfected 293T: (A), human ERGIC-53 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of ERGIC-53 expression in HeLa whole cell lysate (A) and truncated human recombinant ERGIC-53 fusion protein (B).| Application Notes | recommended for detection of ERGIC-53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
