Application
Western blot analysis of P23 expression in Jurkat (A), NIH/3T3 (B), HeLa (C), A549 (D), HS treated HeLa (E) and HS treated NIH/3T3 (F) whole cell lysates.Application
Western blot analysis of P23 expression in 293T whole cell lysate.| Product Name | P23 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 26-85 mapping near the N-terminus of P23 of human origin |
| Modification Notes | P23, also known as PTGES3 (prostaglandin E synthase 3) or TEBP (telomerase-binding protein p23), is a ubiquitously expressed protein that functions as a cochaperone and plays an important role in signal transduction. One of several proteins in the HSP 90-based molecular chaperone complex, P23 promotes the breakdown of transcriptional regulatory complexes by disrupting receptor-mediated transcriptional activation. P23 acts in a hormone-dependent manner to chaperone estrogen receptor alpha (ERå), a steroid complex, to its mature form and to regulate the expression of ERå-related genes. Localized to the cytoplasm, P23 interacts with the glucocorticoid receptor (GR) and, through disassembly of the GR transcription machinery, is thought to inhibit GR-dependent transcription. The involvement of P23 in various steroid receptor-mediated pathways suggests close involvement in signal transduction and regulation of cellular processes. Upregulation of P23 is implicated in the invasion and metastasis of various cancers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of P23 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 26-85 mapping near the N-terminus of P23 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 26-85 mapping near the N-terminus of P23 of human origin |
| Entrez Gene | 10728 |
|---|
Application
Western blot analysis of P23 expression in Jurkat (A), NIH/3T3 (B), HeLa (C), A549 (D), HS treated HeLa (E) and HS treated NIH/3T3 (F) whole cell lysates.
Application
Western blot analysis of P23 expression in 293T whole cell lysate.| Application Notes | recommended for detection of P23 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
