Application
Western blot analysis of Max expression in non-transfected: (A) and human Max transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Max expression in HeLa nuclear extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.Application
Western blot analysis of 20 ng of human recombinant Max (A) and control (B) fusion proteins.| Product Name | Max Antibody (C-124) HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 28-151 mapping at the C-terminus of Max p21 of human origin |
| Modification Notes | It is now well established that the nature and relative abundance of individual subunits of different classes of transcription factors can positively or negatively regulate levels of gene expression. Myc proteins homodimerize and bind DNA poorly, if at all, at physiological levels. Max is a nuclear localized bHLH-Zip protein initially identified by screening a B cell expression library with the bHLH-Zip region of c-Myc. Max homodimers and the Myc-Max heterodimers bind the sequence CACGTG; however the binding of the hetero-dimeric complex is stronger than the Max homodimer. The Max gene products have been identified as Max and Max 9 proteins that differ by a 9 amino acid insertion N-terminal to the basic region. In contrast to Myc, which is highly regulated during progression through the cell cycle, Max is highly stable and is much more abundant than Myc. Two members of the bHLH-Zip protein family, designated Mad and Mxi1, homodimerize poorly but form heterodimeric complexes with Max that have opposing functions to Myc-Max heterodimers with respect to regulation of gene expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Max p21 and p22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 28-151 mapping at the C-terminus of Max p21 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 28-151 mapping at the C-terminus of Max p21 of human origin |
| Entrez Gene | 4149 |
|---|
Application
Western blot analysis of Max expression in non-transfected: (A) and human Max transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Max expression in HeLa nuclear extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
Application
Western blot analysis of 20 ng of human recombinant Max (A) and control (B) fusion proteins.| Application Notes | recommended for detection of Max p21 and p22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
