Application
Western blot analysis of TFIIB expression in HeLa (A), Hep G2 (B) and U-937 (C) nuclear extracts.Application
Western blot analysis of TFIIB expression in non-transfected: (A) and mouse TFIIB transfected: (B) 293T whole cell lysates.Application
ChIP analysis of TFIIB recruitment to genomic amplicons. Two (1,2) different human genomic TBP and three control amplicons (3-5) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with TFIIB (C-18), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with TFIIB (C-18), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Western blot analysis of TFIIB p33 expression in Jurkat (A) and K-562 (B) whole cell lysates.| Product Name | TFIIB Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of TFIIB of human origin |
| Modification Notes | In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the “TATA” element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISAGSChIP |
| recommended for detection of TFIIB p33 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of TFIIB of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of TFIIB of human origin |
| Entrez Gene | 2959 |
|---|
Application
Western blot analysis of TFIIB expression in HeLa (A), Hep G2 (B) and U-937 (C) nuclear extracts.
Application
Western blot analysis of TFIIB expression in non-transfected: (A) and mouse TFIIB transfected: (B) 293T whole cell lysates.
Application
ChIP analysis of TFIIB recruitment to genomic amplicons. Two (1,2) different human genomic TBP and three control amplicons (3-5) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with TFIIB (C-18), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with TFIIB (C-18), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Western blot analysis of TFIIB p33 expression in Jurkat (A) and K-562 (B) whole cell lysates.| Application Notes | recommended for detection of TFIIB p33 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
