Application
Western blot analysis of NFκB p50 expression in non-transfected 293T: (A), mouse NFκB p50 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of NFκB p50 expression in non-transfected: (A) and human NFκB p50 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , HDAC3 (H-99): , HDAC3 (N-19): , Bcl-3 (C-14): , Bcl-3 (H-146): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Western blot analysis of NFκB p50 expression in HeLa whole cell lysate.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic and nuclear staining.Application
Western blot analysis of NFκB p50 and p105 expression in A-431 whole cell lysate.| Product Name | NFκB p50 Antibody (H-119) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 120-239 mapping at the N-terminus of NFκB of human origin |
| Modification Notes | Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF˚B (p50 and p65) and the Drosophila maternal morphagen, dorsal. These proteins share sequence homology over a region of 300 amino acids at their NH2-terminus, the region that contains their DNA binding and dimerization domains. The DNA binding activity of NF˚B is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins have been described. These proteins, designated p105 and p100, are highly related but map on different chromosomes. The p105 (p110) precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated PdI, binds to p50 and regulates its activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of NFκB p50 and p105 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 120-239 mapping at the N-terminus of NF&kappa B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 120-239 mapping at the N-terminus of NFκB of human origin |
| Entrez Gene | 4790 |
|---|
Application
Western blot analysis of NFκB p50 expression in non-transfected 293T: (A), mouse NFκB p50 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of NFκB p50 expression in non-transfected: (A) and human NFκB p50 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , HDAC3 (H-99): , HDAC3 (N-19): , Bcl-3 (C-14): , Bcl-3 (H-146): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Western blot analysis of NFκB p50 expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic and nuclear staining.
Application
Western blot analysis of NFκB p50 and p105 expression in A-431 whole cell lysate.| Application Notes | recommended for detection of NFκB p50 and p105 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
