| Product Name | Skeletrophin Antibody (H-102) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Skeletrophin, also known as MIB2 (Mindbomb homolog 2), SKD, ZZANK1 or ZZZ5, is an E3 ubiquitin-protein ligase that regulates Notch signaling via protein degradation. Expressed in heart, brain, kidney and skeletal muscle, Skeletrophin positively regulates Notch signaling by ubiquitinating the intracellular domain of Delta receptors, which act as ligands for Notch proteins. This ubiquitination facilitates transendocytosis of the Notch extracellular domain which, in turn, cues transportation of the Notch intracellular domain to the nucleus, where it activates a variety of genes. Skeletrophin localizes to the endosome and is down-regulated in primary skin melanomas. Treatment of melanoma cells with 5'-aza-2-deoxycytidine, a demethylating agent, increases Skeletrophin expression, suggesting that down-regulation is caused by methylation of the Skeletrophin gene. Six isoforms exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Skeletrophin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 466-567 mapping within an internal region of Skeletrophin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 466-567 mapping within an internal region of Skeletrophin of human origin |
| Entrez Gene | 142678 |
|---|
| Application Notes | recommended for detection of Skeletrophin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
