Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic vesicles localization.Application
Western blot analysis of ACOT1/2 expression in HeLa (A) and Hep G2 (B) whole cell lysates.| Product Name | ACOT1/2 Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT1 (acyl-CoA thioesterase 1, also known as CTE1) and ACOT2 (acyl-CoA thioesterase 2, also known as PTE2) are members of the ACOT family and exhibit different cellular localization, with ACOT1 existing as a monomer in the cytoplasm and ACOT2 localized primarily to mitochondria. Characteristic of most ACOT proteins, ACOT1 and ACOT2 catalyze the conversion of Palmitoyl-CoA and water to free CoA and palmitate, a reaction that is important for the regulation of intercellular fatty acid levels. ACOT2 is expressed as multiple alternatively spliced isoforms and, like ACOT1, is encoded by a gene which maps to human chromosome 14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ACOT1/2 of human origin by WB, IP, IF and ELISA non cross-reactive with other family members | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 249-324 mapping within an internal region of ACOT1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 249-324 mapping within an internal region of ACOT1 of human origin |
| Entrez Gene | 10965 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic vesicles localization.
Application
Western blot analysis of ACOT1/2 expression in HeLa (A) and Hep G2 (B) whole cell lysates.| Application Notes | recommended for detection of ACOT1/2 of human origin by WB, IP, IF and ELISA non cross-reactive with other family members |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
