Application
Western blot analysis of RYBP expression in non-transfected 293T: (A), human RYBP transfected 293T: (B) and K-562 (C) whole cell lysates.| Product Name | RYBP Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The products of the Polycomb group (PcG) of genes are necessary for the maintenance of transcriptional repression of a number of important developmental genes, including the homeotic genes. RYBP is a member of the mammalian polycomb complex. RYBP (RING1- and YY1-binding protein) interacts specifically with the E2F2 and E2F3 family members, dependent on the marked box domain in these proteins. YY1 and RYBP, in combination with either E2F2 or E2F3, can stimulate Cdc6 promoter activity synergistically, at G1/S of the cell cycle. RYBP also complexes with both RING1 proteins (RING1 and RING1B) and with M33, two mutually interacting sets of proteins of the mammalian Polycomb complex. RING1 binds RYBP and M33 through the same C-terminal domain, whereas the RYBP-M33 interaction takes place through an M33 domain not involved in binding. RYBP is widely expressed with highest levels in lymphoid tissues and placenta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of RING1 and YY1 binding protein of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 89-148 mapping within an internal region of RYBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 89-148 mapping within an internal region of RYBP of human origin |
| Entrez Gene | 23429 |
|---|
Application
Western blot analysis of RYBP expression in non-transfected 293T: (A), human RYBP transfected 293T: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of RING1 and YY1 binding protein of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
