Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of DP-1 expression in non-transfected: (A) and human DP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of DP-1 expression in Daudi whole cell lysate.Application
Western blot analysis of DP-1 expression in A-431 nuclear extract.| Product Name | DP-1 Antibody (K-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the Adeno-virus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 forms heterodimers with a second protein, designated DP-1, forming an “active” E2F transcriptional regulatory complex. Additional members of the E2F family include E2F-2, E2F-3, E2F-4, E2F-5 and DP-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of DP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of DP-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of DP-1 of human origin |
| Entrez Gene | 7027 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of DP-1 expression in non-transfected: (A) and human DP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of DP-1 expression in Daudi whole cell lysate.
Application
Western blot analysis of DP-1 expression in A-431 nuclear extract.| Application Notes | recommended for detection of DP-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
