Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas showing cytoplasmic staining of exocrine glandular cells & islet cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas showing cytoplasmic staining of exocrine glandular cells & islet cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Prohibitin 2 expression in non-transfected: (A) and human Prohibitin 2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Prohibitin 2 expression in MCF7 (A), SK-BR-3 (B) and NIH/3T3 (C) whole cell lysates and human recombinant Prohibitin 2 fusion protein (D).| Product Name | Prohibitin 2 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human Prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that Prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progression in more than 80% of the cell lines analyzed. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Prohibitin 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 220-299 mapping at the C-terminus of Prohibitin 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 220-299 mapping at the C-terminus of Prohibitin 2 of human origin |
| Entrez Gene | 11331 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas showing cytoplasmic staining of exocrine glandular cells & islet cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas showing cytoplasmic staining of exocrine glandular cells & islet cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Prohibitin 2 expression in non-transfected: (A) and human Prohibitin 2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Prohibitin 2 expression in MCF7 (A), SK-BR-3 (B) and NIH/3T3 (C) whole cell lysates and human recombinant Prohibitin 2 fusion protein (D).| Application Notes | recommended for detection of Prohibitin 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
