| Product Name | LAGE-1 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cancer-testis (CT) antigens are expressed in various cancers, and absent in normal adult tissues, thereby functioning as useful vaccine targets for immunotherapy. The LAGE-1 gene gives rise to two CT products: a 180 amino acid protein containing 2 glycine-rich regions and a hydrophobic stretch near the C-terminus, and an alternate 210 amino acid protein that lacks the hydrophobic stretch near the C terminus. Both proteins are expressed in melanoma cell lines, lung tumors, sarcomas, and other types of cancer cells, but but not in normal tissues except for testis and placenta. LAGE-1 activates type-1 melanoma-reactive CD4+ helper-T cells and, thus, promotes long-lasting antitumor responses. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LAGE-1 of human origin by WB, IP, IF and ELISA non cross-reactive with LAGE-1A | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-210 mapping at the C-terminus of LAGE-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-210 mapping at the C-terminus of LAGE-1 of human origin |
| Application Notes | recommended for detection of LAGE-1 of human origin by WB, IP, IF and ELISA non cross-reactive with LAGE-1A |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
