Application
Western blot analysis of phosphorylated SMC1 expression in untreated (A) and UV-treated (B) K-562 cell extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing nuclear localization.| Product Name | p-SMC1 Antibody (Ser 957) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) along with SMC3 (also designated HCAP for human chromosome-associated protein or Bama-can), and SMC2 (also designated hCAP-E) along with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan (called Bamacan) which is expressed in testis and brain. Bamacan contains several glycosylation sites and is thought to play a role in basement membrane physiology. Human and mouse SMC1 are subject to phosphorylation on specific amino acid residues, such as Ser 957, which may activate SMC1 activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 957 phosphorylated SMC1 of mouse and human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Ser 957 phosphorylated SMC1 of mouse and human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 8243 |
|---|
Application
Western blot analysis of phosphorylated SMC1 expression in untreated (A) and UV-treated (B) K-562 cell extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing nuclear localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
