Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of U2AF65 expression in non-transfected 293T: (A), mouse U2AF65 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.Application
Western blot analysis of U2AF65 expression in HeLa (A), SK-N-MC (B) and Jurkat (C) nuclear extracts.| Product Name | U2AF65 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SF3b is an U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAPs 49, 130, 145 and 155. SAPs 130, 145 and 155 associate with one another to form a complex that is present in HeLa nuclear extracts. SAPs 49 and 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA, which suggests that this protein complex plays a role in tethering U2 snRNP to the branch site. U2AF recruits SAP 49 to the branch point sequence during the initial steps of spliceosome assembly. U2AF exists as a heterodimer consisting of U2AF65 and U2AF35 and is required for splicing in vivo. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of U2AF65 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of U2AF65 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of U2AF65 of human origin |
| Entrez Gene | 11338 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of U2AF65 expression in non-transfected 293T: (A), mouse U2AF65 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
Application
Western blot analysis of U2AF65 expression in HeLa (A), SK-N-MC (B) and Jurkat (C) nuclear extracts.| Application Notes | recommended for detection of U2AF65 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
