Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Product Name | Thrombin API Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin, and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) leads to formation of a 36 amino acid thrombin light chain (LC; amino acids 328-363) and a 259 amino acid Thrombin heavy chain (HC; amino acids 364-622). In the first step of the coagulation cascade Thrombin cleaves bonds after Arg-|-Gly and activates factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Thrombin Activation Peptide 1 and Prothrombin of human origin by WB, IP, IF, IHC(P) and ELISA does not detect APII, LC or HC also reactive with additional species, including equine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-155 mapping near the N-terminus of Thrombin Activation Peptide 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-155 mapping near the N-terminus of Thrombin Activation Peptide 1 of human origin |
| Entrez Gene | 2147 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Application Notes | recommended for detection of Thrombin Activation Peptide 1 and Prothrombin of human origin by WB, IP, IF, IHC(P) and ELISA does not detect APII, LC or HC also reactive with additional species, including equine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
