Application
Western blot analysis of TEF-3 expression in non-transfected: (A) and human TEF-3 transfected: (B) 293 whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of human TEF-3 transfected 293T whole cell lysate.| Product Name | TEF-3 Antibody (W-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | TEF-3, also known as TEAD4 (TEA domain family member 4), RTEF1, EFTR-2, TEFR-1, TCF13L1 or hRTEF-1B, is a 427 amino acid member of the transcriptional enhancer factor (TEF) family of proteins that are characterized by the presence of a TEA DNA-binding domain. Localized to the nucleus and expressed primarily in skeletal muscle, TEF-3 functions as a transcriptional regulator by binding specifically and non-cooperatively to the M-CAT motif found in the promotors of muscle-specific genes, thereby directing their subsequent expression. TEF-3 contains one TEA DNA-binding domain and is expressed as multiple isoforms due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TEF-3 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of TEF-3 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 7004 |
|---|
Application
Western blot analysis of TEF-3 expression in non-transfected: (A) and human TEF-3 transfected: (B) 293 whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of human TEF-3 transfected 293T whole cell lysate.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
