Application
Western blot analysis of SEMA3C expression in non-transfected: (A) and human SEMA3C transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SEMA3C expression in non-transfected: (A) and human SEMA3C transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of SEMA3C expression in mouse brain (A), rat brain (B) and rat heart (C) tissue extracts.| Product Name | SEMA3C Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SEMA3C (also designated semaphorin 3C, semaphorin E, SEMAE, SemE, sema domain, immunoglobulin domain (Ig) and short basic domain, secreted) is an ubiquitous protein that mediates axonal guidance, repulsive gradients, induction of growth cone collapse and cell survival/death. Secreted SEMA3C favors survival and neuritogenesis of cultured cerebellar granule neurons (CGNs). SEMA3C from macrophages and fibroblasts that selectively directs against sympathetic nerve fibers may be one element responsible for reduced sympathetic innervation in rheumatoid arthritis tissue. SEMA3C mutant mice die within hours after birth from congenital cardiovascular defects consisting of interruption of the aortic arch and improper septation of the cardiac outflow tract. SEMA3C is expressed in the cardiac outflow tract as neural crest cells and promotes crest cell migration into the proximal cardiac outflow tract. Semaphorins constitute a family of molecules sharing a common extracellular domain (semaphorin domain). The family includes several types of secreted and membrane-associated molecules that are grouped into eight subclasses (subclasses 1-7 and viral semaphorins). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor and mature SEMA3C and to a lesser extent SEMA3D of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 592-751 mapping at the C-terminus of SEMA3C precursor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 592-751 mapping at the C-terminus of SEMA3C precursor of human origin |
| Entrez Gene | 10512 |
|---|
Application
Western blot analysis of SEMA3C expression in non-transfected: (A) and human SEMA3C transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SEMA3C expression in non-transfected: (A) and human SEMA3C transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of SEMA3C expression in mouse brain (A), rat brain (B) and rat heart (C) tissue extracts.| Application Notes | recommended for detection of precursor and mature SEMA3C and to a lesser extent SEMA3D of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
