Application
Western blot analysis of Rsk-1 expression in HeLa whole cell lysate.Application
Western blot analysis of Rsk-1 expression in non-transfected (A) and human Rsk-1 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic localization.| Product Name | Rsk-1 Antibody (W-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development. The family of ribosomal S6 kinases includes p70 S6 kinase and p70 S6 kinase ∫, which are thought to have similar regulatory functions. MSK1 (also designated RLPK) is a novel Rsk-related protein, which, like the p90 Rsk family members, contains two non-identical complete kinase catalytic domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Rsk-1 of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Rsk-1 of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 6195 |
|---|
Application
Western blot analysis of Rsk-1 expression in HeLa whole cell lysate.
Application
Western blot analysis of Rsk-1 expression in non-transfected (A) and human Rsk-1 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
