Application
Western blot analysis of MCT4 expression in HCT 116 (A) and HeLa (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing membrane staining of decidual and thropoblastic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | MCT4 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions (2). However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis (2). In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell (1,2). This transport process is carried out by a family of monocarboxylate transporters (MCTs), which function as proton symports and are stereoselective for L-lactate (1–3).The MCT family consists of at least eight members, MCT 1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm (4). MCT1 is widely expressed and is the major form of MCT in tumor cells and erythrocytes (5,6). MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MCT4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 376-465 mapping within a C-terminal cytoplasmic domain of MCT4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 376-465 mapping within a C-terminal cytoplasmic domain of MCT4 of human origin |
| Entrez Gene | 9123 |
|---|
Application
Western blot analysis of MCT4 expression in HCT 116 (A) and HeLa (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing membrane staining of decidual and thropoblastic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of MCT4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
