Application
Western blot analysis of ALF expression in Jurkat whole cell lysate.| Product Name | STON1-ALF Antibody (G-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | ALF (TFIIA å/∫ like factor), also known as GTF2A1L (general transcription factor IIA 1-like), is a nuclear and cytoplasmic protein that is specifically expressed in haploid spermatids in the testis. ALF is believed to play a role in spermatogenesis, functioning as a transcription factor, and is related to the large å/∫ subunit of TFIIA, existing as the germ cell-specific paralog. Similar to TFIIA-å/∫, ALF associates with the smaller TFIIA subunit, TFIIA-©, and functions to stabilize the interaction between TBP and DNA by binding directly to TBP and DNA (at the TATA box), thus forming a TBP/ALF/TATA complex which mediates the transcriptional output of a gene. STON1, also known as SALF, SBLF, STN1 or Stonin-1, is a 735 amino acid ubiquitously expressed protein that localizes to both the cytoplasm and the membrane and is thought to be involved in the correct operation of endocytic machinery. The genes encoding both STON1 and ALF map to human chromosome 2 and, when co-transcribed, produce a fusion protein known as STONE1-ALF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of STON1-ALF fusion protein of human origin and ALF of mouse and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of STON1-ALF fusion protein of human origin and ALF of mouse and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 286749 |
|---|
Application
Western blot analysis of ALF expression in Jurkat whole cell lysate.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
