Application
Antibodies tested include p-PKC βI (Thr 642): (A, B, C) and PKC βI (E-3): (D, E, F).Application
Western blot analysis of PKC βI phosphorylation in untreated (A), nocodazole treated (B) and lambda protein phosphatase (A) and nocodazole treated (C) K-562 whole cell lysates.Application
Western blot analysis of phosphorylated PKC βI expression in untreated (A) and PMA-treated (B) K-562 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.| Product Name | p-PKC βI Antibody (Thr 642) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (å, ∫I, ∫II and ©) and novel (n) PKC isoforms (∂, é, Ω, ñ and †). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC ∂ and é are independent of Ca2+. On the other hand, nPKC ∂ and é, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 642 phosphorylated PKC &betaI of rat origin, correspondingly phosphorylated Thr 641 of human origin and correspondingly phosphorylated Thr 609 of mouse origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 642 of PKC &beta I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 642 of PKC βI of human origin |
| Entrez Gene | 5579 |
|---|
Application
Antibodies tested include p-PKC βI (Thr 642): (A, B, C) and PKC βI (E-3): (D, E, F).
Application
Western blot analysis of PKC βI phosphorylation in untreated (A), nocodazole treated (B) and lambda protein phosphatase (A) and nocodazole treated (C) K-562 whole cell lysates.
Application
Western blot analysis of phosphorylated PKC βI expression in untreated (A) and PMA-treated (B) K-562 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Thr 642 phosphorylated PKC &betaI of rat origin, correspondingly phosphorylated Thr 641 of human origin and correspondingly phosphorylated Thr 609 of mouse origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
