Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic staining.Application
Antibodies tested include p-Cdc2 p34 (Thr 161): preincubated with cognate phosphorylated peptide (A) and p-Cdc2 p34 (Thr 161): (B,C).| Product Name | p-Cdc2 p34 Antibody (Thr 161) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 161 phosphorylated Cdc2 p34 of mouse, rat and human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 161 of Cdc2 p34 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 161 of Cdc2 p34 of human origin |
| Entrez Gene | 983 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic staining.
Application
Antibodies tested include p-Cdc2 p34 (Thr 161): preincubated with cognate phosphorylated peptide (A) and p-Cdc2 p34 (Thr 161): (B,C).| Application Notes | recommended for detection of Thr 161 phosphorylated Cdc2 p34 of mouse, rat and human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
