Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | p-ATR Antibody (Ser 428) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the PIK (phosphatidylinositol kinase)-related kinase family are high molecular weight kinases involved in cell cycle progression, DNA recombination and detection of DNA damage. One member of the PI 3-/PI 4-kinase family is ATR (ataxia-telangiectasia- and Rad3-related), also known as FRP1 (for FRAP-related protein 1). ATR is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. ATR is also closely related to three of the family members involved in checkpoint function: Mei-41 (Drosophila), Mec1p (S. cerevisiae) and Rad3 (S.pombe), and as such may be the functional human counterpart of these proteins. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins Rad17 and Rad9, as well as tumor suppressor protein BRCA1. In addition, ATR is essential for early embryonic development. The protein encoded by the human ATR gene localizes to intranuclear foci after DNA damage or inhibition of replication. ATR is phosphorylated at serine residues 428, 435 and 919 and threonine residue 1989. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Ser 428 phosphorylated ATR of human origin and correspondingly Ser 431 phosphorylated ATR of mouse origin of m and h origin by WB, IF and ELISA also reactive with additional species, including equine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 428 of ATR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 428 of ATR of human origin |
| Entrez Gene | 545 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of Ser 428 phosphorylated ATR of human origin and correspondingly Ser 431 phosphorylated ATR of mouse origin of m and h origin by WB, IF and ELISA also reactive with additional species, including equine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
